The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 25, 2023

Filed:

Sep. 22, 2020
Applicant:

Tongji University, Shanghai, CN;

Inventors:

Bin Dong, Shanghai, CN;

Xin Li, Shanghai, CN;

Assignee:

TONGJI UNIVERSITY, Shanghai, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 13/02 (2006.01); C08G 69/08 (2006.01); C12N 1/20 (2006.01); C12R 1/07 (2006.01);
U.S. Cl.
CPC ...
C12P 13/02 (2013.01); C08G 69/08 (2013.01); C12N 1/205 (2021.05); C12R 2001/07 (2021.05);
Abstract

A regulation method for preparing γ-polyglutamic acid by sludge substrate fermentation includes: 1) extraction of glutamic acid from sludge protein (high pressure hydrothermal treatment, gravity pressure filtration treatment), 2) secondary metabolic synthesis of γ-polyglutamic acid (activation of domesticated strains and secondary metabolic fermentation strains); and 3) preparation of pure γ-polyglutamic acid (acidification, centrifugation, filtration, precipitation based on polar repulsion, purification, impurity removal and drying). The present invention realizes a recycling of high-value carbon and nitrogen sources of sludge without secondary pollution, and has advantages of simplified operation, good feasibility, and low preparation cost. The synthesized γ-polyglutamic acid has high economic value and broad application prospect.


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