The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 18, 2023

Filed:

Dec. 16, 2020
Applicant:

Applied Materials, Inc., Santa Clara, CA (US);

Inventors:

Yun-Ching Chang, San Jose, CA (US);

Dan Xie, Dublin, CA (US);

Chloe Kim, San Jose, CA (US);

Assignee:

Applied Materials, Inc., Santa Clara, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/6841 (2018.01); G01N 21/64 (2006.01); G02B 21/00 (2006.01); G02B 21/16 (2006.01); G02B 21/36 (2006.01); G06T 5/00 (2006.01); G06T 5/20 (2006.01); G06T 5/50 (2006.01); G06T 7/00 (2017.01); G16B 50/40 (2019.01); G06V 10/30 (2022.01); G06V 10/75 (2022.01); G06V 20/69 (2022.01); G01N 21/75 (2006.01);
U.S. Cl.
CPC ...
G01N 21/6458 (2013.01); C12Q 1/6841 (2013.01); G01N 21/6428 (2013.01); G01N 21/6456 (2013.01); G02B 21/0076 (2013.01); G02B 21/16 (2013.01); G02B 21/365 (2013.01); G02B 21/367 (2013.01); G06T 5/003 (2013.01); G06T 5/006 (2013.01); G06T 5/20 (2013.01); G06T 5/50 (2013.01); G06T 7/0012 (2013.01); G06V 10/30 (2022.01); G06V 10/758 (2022.01); G06V 20/698 (2022.01); G16B 50/40 (2019.02); G01N 2021/6419 (2013.01); G01N 2021/6421 (2013.01); G01N 2021/6441 (2013.01); G01N 2021/755 (2013.01); G01N 2201/12761 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/30004 (2013.01); G06T 2207/30168 (2013.01);
Abstract

In a fluorescent in-situ hybridization imaging system performs, as nested loops, the following: (1) a valve sequentially couples a flow cell to a plurality of different reagent sources to expose the sample to a plurality of different reagents, (2) for each reagent of the plurality of different reagents, a motor sequentially positions the fluorescence microscope relative to sample at a plurality of different fields of view, (3) for each field of view of the plurality of different fields of view, a variable frequency excitation light source sequentially emits a plurality of different wavelengths, (4) for each wavelength of the plurality of different wavelengths, an actuator sequentially positions the fluorescence microscope relative to sample at a plurality of different vertical heights, and (5) for each vertical height of the plurality of different vertical heights, an image is obtained.


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