The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 18, 2023

Filed:

Nov. 22, 2019
Applicant:

Tempus Labs, Inc., Chicago, IL (US);

Inventors:

Ameen Salahudeen, Oak Park, IL (US);

Verónica Sánchez Freire, Chicago, IL (US);

Brandon L. Mapes, Chicago, IL (US);

Assignee:

Tempus Labs, Inc., Chicago, IL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12P 19/34 (2006.01); C12Q 1/6886 (2018.01); C12N 5/071 (2010.01); G16H 50/30 (2018.01); C12M 1/32 (2006.01); G01N 33/50 (2006.01); G16B 20/20 (2019.01); C12N 5/09 (2010.01); C12Q 1/02 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6816 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6869 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6886 (2013.01); C12M 23/12 (2013.01); C12N 5/0691 (2013.01); C12N 5/0693 (2013.01); C12Q 1/025 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6816 (2013.01); G01N 33/5091 (2013.01); G16B 20/20 (2019.02); G16H 50/30 (2018.01); C12N 2500/25 (2013.01); C12N 2501/11 (2013.01); C12N 2501/117 (2013.01); C12N 2501/119 (2013.01); C12N 2501/33 (2013.01); C12N 2501/998 (2013.01); C12N 2501/999 (2013.01); C12N 2509/10 (2013.01); C12N 2521/10 (2013.01); C12N 2527/00 (2013.01); C12N 2533/52 (2013.01); C12N 2533/54 (2013.01); C12N 2533/90 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/6869 (2013.01);
Abstract

Provided herein are novel organoid culture media, organoid culture systems, and methods of culturing tumor organoids using the subject organoid culture media. Also provided herein are tumor organoids developed using such organoid culture systems, methods for assessing the clonal diversity of the tumor organoids, and methods for using such tumor organoids, for example, for tumor modelling and drug development applications. In particular embodiments, the tumor organoid culture media provided herein is substantially free of R-spondins (e.g., R-spondin1).


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