The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 18, 2023

Filed:

Jun. 22, 2022
Applicant:

Jiangnan University, Wuxi, CN;

Inventors:

Jing Hu, Wuxi, CN;

Jian Yin, Wuxi, CN;

Xiang Wang, Wuxi, CN;

Assignee:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/077 (2010.01); C08J 3/075 (2006.01); A23L 13/00 (2016.01); C12N 9/10 (2006.01); A23L 17/00 (2016.01);
U.S. Cl.
CPC ...
C12N 5/0658 (2013.01); C08J 3/075 (2013.01); C12N 9/1044 (2013.01); A23L 13/00 (2016.08); A23L 17/00 (2016.08); C08J 2305/04 (2013.01); C08J 2405/04 (2013.01); C08J 2405/10 (2013.01); C12N 2533/54 (2013.01); C12N 2533/74 (2013.01); C12N 2537/10 (2013.01);
Abstract

Described is a crosslinked hydrogel for muscle stem cell culture and a preparation method and use thereof. The preparation method includes: dissolving collagen to prepare a solution and adding alginate and heparan sulfate proteoglycan and uniformly mixing with the collagen solution; adding ε-PL and TGase into the solution, uniformly stirring, and putting a slurry into a mold for crosslinking to obtain the hydrogel. The hydrogel is prepared by linking the collagen, the polylysine, and the heparan sulfate proteoglycan using the TGase to form covalent crosslinking, and forming a compact three-dimensional 'egg box' network structure through a physical electrostatic interaction between the polylysine and the alginate.


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