The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 11, 2023
Filed:
Sep. 07, 2022
Shandong University, Shandong, CN;
Shandong Institute for Food and Drug Control, Shandong, CN;
Lianli Chi, Shandong, CN;
Deling Shi, Shandong, CN;
Bin Zhang, Shandong, CN;
Feng Shi, Shandong, CN;
Mengmeng Li, Shandong, CN;
Baojian Hang, Shandong, CN;
Shandong University, Shandong, CN;
Shandong Institute for Food and Drug Control, Shandong, CN;
Abstract
The present application is related to a method for identifying whether porcine heparin is adulterated with heparin from ruminants, comprising: (1) respectively detecting the contents of trisaccharide(4S) and ΔUA2S-GlcNAc6S (ΔIA) in a sample and at least three batches of porcine heparin standards; (2) calculating a ratio of the trisaccharide(4S) to the ΔIA as well as a standard deviation (SD) of the ratio in the porcine heparin standards; when the ratio of the trisaccharide(4S) to the ΔIA in the sample exceeds a maximum value of the ratio in the porcine heparin standards+3SD, where the sample is considered to be mixed or adulterated with heparin from ruminants; wherein the detection method used is hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-MS) or multiple reaction monitoring (MRM). The method can distinguish porcine heparin from ovine and bovine heparin based on the structural differences, regardless of the production process the heparin has undergone.