The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 11, 2023
Filed:
Aug. 08, 2017
Applicants:
Peking University, Beijing, CN;
Edigene Biotechnology Inc., Beijing, CN;
Inventors:
Wensheng Wei, Beijing, CN;
Yiou Chen, Beijing, CN;
Yuexin Zhou, Beijing, CN;
Hongmin Zhang, Beijing, CN;
Pengfei Yuan, Beijing, CN;
Yuan Liu, Beijing, CN;
Assignees:
Peking University, Beijing, CN;
Edigene Biotechnology Inc., Beijing, CN;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/90 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/65 (2006.01); G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
C12N 15/90 (2013.01); C12N 9/22 (2013.01); C12N 15/111 (2013.01); C12N 15/65 (2013.01); G01N 21/6428 (2013.01); C12N 2310/3517 (2013.01);
Abstract
A donor construct and a gene knockout method, as well as a system and kit for the gene knockout are provided. The donor construct is a linear donor DNA or can be cleaved in a cell to produce the linear donor DNA. The gene knockout method uses a marker gene contained in the donor construct to enrich cells in which a gene is knocked out, thereby improving the efficiency of generating the gene knockout by a sequence-specific nuclease.