The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 11, 2023

Filed:

Apr. 30, 2018
Applicant:

Agrospheres, Inc., Charlottesville, VA (US);

Inventors:

Ameer Hamza Shakeel, Leesburg, VA (US);

Zachery George Davis, Charlottesville, VA (US);

Joseph Thomas Frank, Charlottesville, VA (US);

Sepehr Zomorodi, Charlottesville, VA (US);

Payam Pourtaheri, Charlottesville, VA (US);

Assignee:

AgroSpheres, Inc., Charlottesville, VA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 11/16 (2006.01); C07K 14/005 (2006.01); C07K 14/21 (2006.01); C07K 14/415 (2006.01); C12N 1/20 (2006.01); C12N 9/20 (2006.01); C12N 9/86 (2006.01); C12N 15/70 (2006.01); C12N 15/74 (2006.01); B82Y 5/00 (2011.01);
U.S. Cl.
CPC ...
C12N 11/16 (2013.01); C07K 14/005 (2013.01); C07K 14/21 (2013.01); C07K 14/415 (2013.01); C12N 1/20 (2013.01); C12N 9/20 (2013.01); C12N 9/86 (2013.01); C12N 15/70 (2013.01); C12N 15/74 (2013.01); B82Y 5/00 (2013.01); C07K 2319/03 (2013.01); C07K 2319/735 (2013.01); C12Y 301/01034 (2013.01); C12Y 301/03001 (2013.01); C12Y 305/02006 (2013.01);
Abstract

The present disclosure relates to compositions of immobilized enzymes on the surface of achromosomal and/or anucleate cells and uses thereof. In particular, the present disclosure provides genetically engineered minicells with enzymes self-assembled on their surface. The immobilized enzymes on the surface of achromosomal and/or anucleate minicells, has agricultural, industrial, and environmental applications due to their improved stability durability and, reusability. Also, provided are methods for producing and purifying enzyme-immobilized minicells.


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