The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 04, 2023
Filed:
Jun. 22, 2017
Applicant:
Shimadzu Corporation, Kyoto, JP;
Inventors:
Noriko Iwamoto, Kyoto, JP;
Takashi Shimada, Kyoto, JP;
Assignee:
SHIMADZU CORPORATION, Kyoto, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 30/72 (2006.01); G01N 33/68 (2006.01); C12Q 1/37 (2006.01); G01N 30/88 (2006.01);
U.S. Cl.
CPC ...
G01N 30/7233 (2013.01); C12Q 1/37 (2013.01); G01N 33/6848 (2013.01); G01N 33/6857 (2013.01); G01N 2030/8818 (2013.01); G01N 2333/976 (2013.01); G01N 2560/00 (2013.01); G01N 2800/52 (2013.01);
Abstract
The present invention provides a method in which a porous body having a monoclonal antibody to be measured immobilized in pores thereof is brought into contact with nanoparticles having a protease immobilized thereonto in a liquid to perform selective protease digestion of the monoclonal antibody and a peptide fragment obtained by the digestion is detected by liquid chromatography mass spectrometry (LC-MS), wherein the monoclonal antibody is digested with the protease in the presence of an antibody specifically binding to the monoclonal antibody or a target molecule of the monoclonal antibody.