The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 04, 2023
Filed:
May. 02, 2022
Michael Edward Hogan, Tucson, AZ (US);
Benjamin Alan Katchman, Tucson, AZ (US);
Candy Mavis Rivas, Tucson, AZ (US);
Yasmine Eve Baca, San Tan Valley, AZ (US);
Michael Edward Hogan, Tucson, AZ (US);
Benjamin Alan Katchman, Tucson, AZ (US);
Candy Mavis Rivas, Tucson, AZ (US);
Yasmine Eve Baca, San Tan Valley, AZ (US);
PathogenDx, Inc., Scottsdale, AZ (US);
Abstract
Provided herein are methods for isolating nucleic acids from intact cells in a sample of intact cells and cell free nucleic acids. The intact cells and cell free nucleic acids are captured and concentrated within the pores of a silicate matrix in a microporous silicate filter. Within the silicate matrix the cell free nucleic acids are degraded with a nuclease, the intact cells are lysed with the released DNA binding to the silicate, the nuclease treated cell free nucleic acids and contaminants from the lysed are washed from the silicate matrix and the DNA bound to the silicate is eluted therefrom to form an isolated DNA product.