The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 04, 2023
Filed:
Dec. 10, 2020
Jiangnan University, Wuxi, CN;
Long Liu, Wuxi, CN;
Jian Chen, Wuxi, CN;
Guocheng Du, Wuxi, CN;
Jianghua Li, Wuxi, CN;
Xueqin Lv, Wuxi, CN;
Li Sun, Wuxi, CN;
JIANGNAN UNIVERSITY, Jiangsu, CN;
Abstract
The present invention provides an acid-tolerantstrain and use thereof. By using exogenously added malic acid as a stress, an acid-tolerant mutantstrain MTPfo-4 is obtained by directed evolution screening in the laboratory, which tolerates a minimum pH of 2.44. The mutant strain MTPfo-4, tolerant to multiple organic acids, has an increased tolerance to exogenous malic acid of up to 86.6 g/L. The mutant strain MTPfo-4 obtained is further identified. The mutant strain grows stably and well, and can tolerate a variety of organic acids (lactic acid, malic acid, succinic acid, fumaric acid, citric acid, gluconic acid, and tartaric acid). It also has a strong tolerance to inorganic acids (HCl and HPO). This is difficult to achieve in the existing research and reports of. The strain is intended to be used as an acid-tolerant chassis cell factory for producing various short-chain organic acids.