The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 04, 2023

Filed:

Feb. 16, 2021
Applicant:

Jiangnan University, Wuxi, CN;

Inventors:

Yanfeng Liu, Wuxi, CN;

Long Liu, Wuxi, CN;

Xiaolong Zhang, Wuxi, CN;

Guocheng Du, Wuxi, CN;

Jianghua Li, Wuxi, CN;

Jian Chen, Wuxi, CN;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 1/20 (2006.01); C12N 15/75 (2006.01); C12P 19/26 (2006.01); C12R 1/25 (2006.01);
U.S. Cl.
CPC ...
C12N 15/75 (2013.01); C12N 1/205 (2021.05); C12P 19/26 (2013.01); C12R 2001/25 (2021.05);
Abstract

The disclosure disclosesfor producing N-acetylneuraminic acid and application thereof, and belongs to the field of genetic engineering. The disclosure optimizes the expression levels of key enzymes in N-acetylneuraminic acid synthesis pathways on genome through promoters of different strength, reduces the protein synthesis pressure caused by the expression of enzymes on cells, and further integrates the three N-acetylneuraminic acids in a sameengineering strain.with improved N-acetylneuraminic acid production is obtained, and the production reaches 10.4 g/L at the shake flask level, laying a foundation for further improving the NeuAc production from


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