The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 04, 2023

Filed:

Oct. 26, 2018
Applicant:

Guy Marti, Montigny-sur-Loing, FR;

Inventors:

Guy Marti, Montigny-sur-Loing, FR;

Frank Lay, Baltimore, MD (US);

John W. Harmon, Baltimore, MD (US);

Louis Joseph Born, Pasadena, MD (US);

Zahra Alikhassy Habibabady, Baltimore, MD (US);

Aurelien Markus Marti, Moret-Loing-et-Orvanne, FR;

Assignee:

Other;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/0775 (2010.01); C12M 1/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0667 (2013.01); C12M 45/05 (2013.01);
Abstract

A method of purifying adipose-derived mesenchymal stem cells from a sample of adipose tissue, including: flowing the sample onto a polymer surface having at least one vertical helical-shaped portion, vertical threaded shaped portion, or vertical grooved shaped portion at a first flow rate of 10 to 150 ml/min allowing separation into a first remaining sample including mesenchymal stem cells on the polymer surface and into a second resulting solution being evacuated from the polymer surface; flowing a saline solution onto the polymer surface at a second flow rate of 100 to 500 ml/min, the first flow rate being slower than the second flow rate; and collecting the saline solution including purified mesenchymal stem cells in a collector. Also, an apparatus and a system for purifying adipose-derived mesenchymal stem cells, the use of the apparatus, and a method of isolating and purifying adipose-derived mesenchymal stem cells from an adipose tissue sample.


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