The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 04, 2023
Filed:
Jul. 29, 2016
Shigeyuki Yokoyama, Tokyo, JP;
Shigeyuki Yokoyama, Saitama, JP;
Takehiro Shinoda, Saitama, JP;
Kaori Ito, Saitama, JP;
Naoko Nomura, Saitama, JP;
Yoshiko Katsura, Saitama, JP;
Tomomi Someya, Saitama, JP;
Mikako Shirouzu, Saitama, JP;
Tetsuya Hori, Saitama, JP;
Yoshihiro Nakamura, Saitama, JP;
Hiroaki Tanabe, Saitama, JP;
Other;
Abstract
In order to provide a membrane protein production method which does not require the step of solubilizing a membrane protein and which allows the membrane protein having an excellent quality to be obtained with a high yield, a method in accordance with an embodiment of the present invention includes: a step (a) of preparing a reaction solution for cell-free protein synthesis, the reaction solution containing (i) a template nucleic acid which encodes the membrane protein, (ii) a lipid, and (iii) a detergent which is contained at a concentration equal to or higher than a critical micelle concentration; and a step (b) of synthesizing the membrane protein while the concentration of the detergent in the reaction solution is maintained at a concentration equal to or higher than a critical micelle concentration.