The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 28, 2023
Filed:
Jun. 11, 2021
Applicant:
Fullstem Co., Ltd., Okinawa, JP;
Inventor:
Shunmei Chiba, Okinawa, JP;
Assignee:
FULLSTEM CO., LTD., Okinawa, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/0775 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0667 (2013.01); C12N 5/0663 (2013.01); C12N 2500/34 (2013.01); C12N 2513/00 (2013.01); C12N 2533/00 (2013.01);
Abstract
A method of efficiently recovering a large amount of exosomes from mesenchymal stem cells is provided. The method includes: a three dimensional culture step of three dimensionally culturing mesenchymal stem cells in a medium containing sugar by using a nonwoven fabric as a scaffold; a post-plateau culture step of further culturing for a certain period of time after the amount of the sugar consumed by the mesenchymal stem cells reaches a plateau; and an exosome recovery step of recovering exosomes from the mesenchymal stem cells. The mesenchymal stem cells are adipose-derived mesenchymal stem cells.