The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 21, 2023

Filed:

May. 30, 2016
Applicant:

Curevac SE, Tübingen, DE;

Inventors:

Tilmann Roos, Kusterdingen, DE;

Benyamin Yazdan Panah, Tübingen, DE;

Markus Conzelmann, Tübingen, DE;

Andreas Thess, Kusterdingen, DE;

Dominik Buob, Tübingen, DE;

Martin Kunze, Rottenburg, DE;

Veronika Wagner, Ellwangen, DE;

Assignee:

CureVac SE, Tübingen, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 11/02 (2006.01); C12N 9/12 (2006.01); C12P 19/34 (2006.01); C12N 9/10 (2006.01); C12N 9/16 (2006.01); C12N 11/087 (2020.01); C12N 11/098 (2020.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C12N 9/1007 (2013.01); C12N 9/1241 (2013.01); C12N 9/16 (2013.01); C12N 11/02 (2013.01); C12N 11/087 (2020.01); C12N 11/098 (2020.01); C12Y 201/01056 (2013.01); C12Y 201/01057 (2013.01); C12Y 207/0705 (2013.01); C12Y 301/03033 (2013.01);
Abstract

The present invention relates to an immobilized capping enzyme, preferably an immobilized Vaccinia virus capping enzyme. Furthermore, the present invention relates to an immobilized cap-specific nucleoside 2'-O-methyltransferase, preferably an immobilized Vaccinia virus cap-specific nucleoside 2′-O-methyltransferase. Moreover, the present invention relates to a method for immobilizing said enzymes and to a method of using said enzymes for the addition of a 5′-cap structure to RNAs. Moreover, the present invention relates to an enzyme reactor for performing the capping reaction using said immobilized enzymes and the subsequent separation of the 5′-capped RNA product. In addition, the present invention relates to a kit comprising the capping enzyme and/or the cap-specific nucleoside 2′-O-methyltransferase.


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