The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 21, 2023

Filed:

Nov. 07, 2017
Applicant:

Becton, Dickinson and Company, Franklin Lakes, NJ (US);

Inventors:

Jing Hu, Menlo Park, CA (US);

Christina Fan, Menlo Park, CA (US);

David Rosenfeld, Menlo Park, CA (US);

Jue Fan, Menlo Park, CA (US);

Elisabeth Marie Walczak, Menlo Park, CA (US);

Assignee:

Becton, Dickinson And Company, Franklin Lakes, NJ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); G16B 45/00 (2019.01); C12Q 1/6869 (2018.01); G16B 20/00 (2019.01);
U.S. Cl.
CPC ...
C12N 15/1065 (2013.01); C12Q 1/6869 (2013.01); G16B 20/00 (2019.02); G16B 45/00 (2019.02); C12Q 2525/161 (2013.01); C12Q 2535/122 (2013.01);
Abstract

Disclosed herein are methods and systems for classifying cell labels, for example identifying a signal cell label. In some embodiments, the method comprises: obtaining sequencing data of barcoded targets created using targets in cells barcoded using barcodes, wherein a barcode comprises a cell label and a molecular label. After ranking the cell labels, a minimum of a second derivative plot of a cumulative sum plot can be determined. Using the methods, a cell label can be classified as a signal cell label or a noise cell label based on the number of molecular labels with distinct sequences associated with the cell label and a cell label threshold.


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