The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 14, 2023

Filed:

Aug. 17, 2018
Applicant:

Asymchem Life Science (Tianjin) Co., Ltd, Tianjin, CN;

Inventors:

Hao Hong, Morrisville, NC (US);

Gage James, Morrisville, NC (US);

Jiangping Lu, Tianjin, CN;

Na Zhang, Tianjin, CN;

Xuecheng Jiao, Tianjin, CN;

Rui Li, Tianjin, CN;

Kejian Zhang, Tianjin, CN;

Yu Zhang, Tianjin, CN;

Yiming Yang, Tianjin, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/06 (2006.01); C12N 15/70 (2006.01); C12P 13/04 (2006.01); C12R 1/19 (2006.01);
U.S. Cl.
CPC ...
C12N 9/0012 (2013.01); C12N 15/70 (2013.01); C12P 13/04 (2013.01); C12R 2001/19 (2021.05); C12Y 104/99001 (2013.01);
Abstract

Provided is an amino acid dehydrogenase mutant. The amino acid sequence of the mutant is obtained by mutating the amino acid sequence shown in SEQ ID NO:1. The mutation includes at least one of the following mutation sites: 64th, 94th, 133rd, 137th, 148th, 168th, 173rd, 183 rd, 191st, 207th, 229th, 248th, 255th and 282nd sites; or the amino acid sequence of the amino acid dehydrogenase mutant is an amino acid sequence having the mutation sites in the mutated amino acid sequence and having a 80% or more homology with the mutated amino acid sequence. The mutant enzyme activity is more than 50 times higher than that of wild amino acid dehydrogenase, and the enzyme specificity is also correspondingly improved.


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