The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 14, 2023

Filed:

Jun. 11, 2021
Applicant:

Public University Corporation Yokohama City University, Yokohama, JP;

Inventors:

Takanori Takebe, Yokohama, JP;

Hideki Taniguchi, Yokohama, JP;

Yoshinobu Takahashi, Yokohama, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01K 67/027 (2006.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01); G01N 33/50 (2006.01); C07D 499/21 (2006.01); A61L 27/38 (2006.01); A61K 35/28 (2015.01); A61K 35/44 (2015.01); A61K 49/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0691 (2013.01); A01K 67/0271 (2013.01); A61K 35/28 (2013.01); A61K 35/44 (2013.01); A61K 49/0008 (2013.01); A61L 27/3808 (2013.01); A61L 27/3834 (2013.01); A61L 27/3886 (2013.01); C07D 499/21 (2013.01); C12N 5/0062 (2013.01); C12N 5/0677 (2013.01); G01N 33/5082 (2013.01); A01K 2207/12 (2013.01); A01K 2227/105 (2013.01); A01K 2267/035 (2013.01); A01K 2267/0325 (2013.01); A01K 2267/0331 (2013.01); A01K 2267/0362 (2013.01); C12N 2502/13 (2013.01); C12N 2506/1346 (2013.01); C12N 2506/28 (2013.01); C12N 2513/00 (2013.01);
Abstract

The present invention provides a method of constituting a tissue construct in vitro using a tissue without depending on scaffold materials. A method of integrating a biological tissue with a vascular system in vitro, comprising coculturing a biological tissue with vascular cells and mesenchymal cells. A biological tissue which has been integrated with a vascular system by the above-described method. A method of preparing a tissue or an organ, comprising transplanting the biological tissue described above into a non-human animal and differentiating the biological tissue into a tissue or an organ in which vascular networks have been constructed. A method of regeneration or function recovery of a tissue or an organ, comprising transplanting the biological tissue described above into a human or a non-human animal and differentiating the biological tissue into a tissue or an organ in which vascular networks have been constructed. A method of preparing a non-human chimeric animal, comprising transplanting the biological tissue described above into a non-human animal and differentiating the biological tissue into a tissue or organ in which vascular networks have been constructed. A method of evaluating a drug, comprising using at least one member selected from the group consisting of the biological tissue described above, the tissue or organ prepared by the method described above, and the non-human chimeric animal prepared by the method described above. A composition for regenerative medicine, comprising a biological tissue which has been integrated with a vascular system by the method described above.


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