Biological substance quantification method, image processing device, pathological diagnosis support system, and recording medium storing computer readable program

Abstract

A biological substance quantification method of quantifying an expression amount of a specific biological substance in a target sample stained by using a fluorescent dye accumulated particle bondable to the specific biological substance. The method includes: inputting a first fluorescent image obtained by image capturing of the target sample; extracting a bright spot portion from the first fluorescent image and calculating a first luminance value; and calculating a number of the fluorescent dye accumulated particle included in the bright spot portion by using the first luminance value, a second luminance value of a bright spot portion extracted from a second fluorescent image obtained by image capturing of a standard sample, and a distribution of a third luminance value of each bright spot portion in a third fluorescent image obtained by image capturing of a preparation on which the fluorescent dye accumulated particle is dispersed without aggregating.