The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 07, 2023
Filed:
Dec. 23, 2019
Applicant:
Nanjing Zhongkeyouzi Institute of Biotechnology Co., Ltd., Jiangsu, CN;
Inventors:
Assignee:
NANJING ZHONGKEYOUZI INSTITUTE OF BIOTECHNOLOGY CO., LTD, Jiangsu, CN;
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12N 15/11 (2006.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C12N 15/11 (2013.01);
Abstract
The invention relates to a plasmid, a DNA assembly method and its application recombinant strain. The plasmid has single adjacent Type IIP and Type IIS RE recognition sites. The plasmid combines the properties of Type IIP and Type IIS REs to achieve recursive cycling, SCAR-free and repeat sequence assembly.