The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 07, 2023

Filed:

Jun. 29, 2018
Applicant:

Zimitech, Inc., Berkeley, CA (US);

Inventors:

Kulika Chomvong, Pathum Thani, TH;

James Harrison Doudna Cate, Berkeley, CA (US);

Yong-Su Jin, Champaign, IL (US);

Assignee:

Zimitech, Inc., Alameda, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/18 (2006.01); C12N 1/19 (2006.01); C12N 15/81 (2006.01); C12N 9/10 (2006.01); C12N 15/54 (2006.01); C07K 14/395 (2006.01); C12N 9/14 (2006.01); C12P 19/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/81 (2013.01); C07K 14/395 (2013.01); C12N 9/14 (2013.01); C12P 19/00 (2013.01); C12Y 306/01003 (2013.01);
Abstract

Disclosed herein are genetically modified microorganisms and related methods for enhanced utilization of oligosaccharides and improved productivity of compounds derived from the metabolism of the oligosaccharides. The microorganisms described herein have altered activities of plasma membrane ATPase protein (PMA1) and/or one or more extracellular glucose sensors, namely, sucrose non-fermenting protein (SNF3), restores glucose transport protein (RGT2), and G protein-coupled receptor 1 protein (GPR1). These genetic modifications provide the microorganisms an increased ability to utilize an oligosaccharide to produce a compound of interest, particularly, tagatose, 2'-fucosyllactose, and psicose. Methods of culturing the microorganisms in the presence of such oligosaccharides to produce the products of interest are also provided.


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