The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 28, 2023

Filed:

Feb. 26, 2020
Applicant:

Indian Institute of Science, Bangalore, IN;

Inventors:

Pallavi Dasgupta, Bengaluru, IN;

Patnam Krishnaswamy, Bengaluru, IN;

Vinay Kumar, Sanjaynagar, IN;

Navakanta Bhat, Bengaluru, IN;

Assignee:

Indian Institute of Science, Bangalore Karnataka, IN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/487 (2006.01); G01N 27/327 (2006.01); G01N 27/333 (2006.01); G01N 33/49 (2006.01); G01N 33/493 (2006.01);
U.S. Cl.
CPC ...
G01N 33/48785 (2013.01); G01N 27/3272 (2013.01); G01N 27/3275 (2013.01); G01N 27/3335 (2013.01); G01N 33/49 (2013.01); G01N 33/493 (2013.01);
Abstract

Methods and devices for quantifying creatinine in a test sample are provided. The test sample is contacted with a sensing composition to obtain a product comprising a hydantoin-transition metal complex and ammonia. The sensing composition comprises creatinine deaminase and a transition metal salt. The creatinine deaminase enzymatically reacts with creatinine to provide the N-methyl hydantoin and ammonia. The N-methyl hydantoin forms the hydantoin-transition metal complex with the transition salt. A potential difference is applied to the product to measure a current signal provided by the hydantoin-transition metal complex. Concentration of N-methyl hydantoin is obtained based on the measured current signal using a calibration equation. The concentration of N-methyl hydantoin is correlated with concentration of creatinine to quantify the creatinine in the test sample.


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