The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 28, 2023
Filed:
Nov. 08, 2021
Applicant:
Gigagen, Inc., South San Francisco, CA (US);
Inventors:
David Scott Johnson, San Francisco, CA (US);
Everett Hurteau Meyer, Redwood City, CA (US);
Assignee:
GigaGen, Inc., South San Francisco, CA (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6874 (2018.01); C12N 15/10 (2006.01); C07K 16/00 (2006.01); C12Q 1/6844 (2018.01); C12Q 1/6886 (2018.01); C12Q 1/6881 (2018.01); C12Q 1/6837 (2018.01); C12Q 1/6883 (2018.01); C12Q 1/6888 (2018.01); C40B 50/06 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6874 (2013.01); C07K 16/00 (2013.01); C12N 15/1006 (2013.01); C12N 15/1065 (2013.01); C12N 15/1075 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6846 (2013.01); C12Q 1/6881 (2013.01); C12Q 1/6883 (2013.01); C12Q 1/6886 (2013.01); C12Q 1/6888 (2013.01); C07K 2317/622 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01); C40B 50/06 (2013.01);
Abstract
Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.