The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 28, 2023
Filed:
Dec. 23, 2019
Applicant:
Cheng-yao Chen, Hsinchu, TW;
Inventor:
Cheng-Yao Chen, Hsinchu, TW;
Assignee:
Other;
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C12Y 207/07007 (2013.01);
Abstract
A method for synthesizing a nucleic acid includes providing an initiator having a 3' end having an unprotected hydroxyl group, providing a nucleic acid polymerase having at least a conservative catalytic polymerase domain of a family-B DNA polymerase, providing a nucleotide monomer, and exposing the initiator to the nucleotide monomer in the presence of the nucleic acid polymerase and a metal cofactor which is a bivalent cation, and in the absence of a template, such that the nucleotide monomer is incorporated to the initiator. The kit includes the initiator, the nucleic acid polymerase, and the nucleotide monomer, and is used according to the method.