The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 28, 2023

Filed:

Mar. 06, 2020
Applicant:

Roche Innovation Center Copenhagen A/s, Hørsholm, DK;

Inventors:

Dennis Jul Hansen, Hørsholm, DK;

Joerg Hoernschemeyer, Basel, CH;

Jacob Ravn, Hørsholm, DK;

Christoph Rosenbohm, Hørsholm, DK;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/11 (2006.01); C07H 21/00 (2006.01); C07H 1/00 (2006.01); C12N 15/113 (2010.01);
U.S. Cl.
CPC ...
C12N 15/111 (2013.01); C07H 1/00 (2013.01); C07H 21/00 (2013.01); C12N 15/113 (2013.01); C12N 2310/311 (2013.01); C12N 2310/312 (2013.01); C12N 2310/314 (2013.01); C12N 2310/321 (2013.01); C12N 2310/3231 (2013.01); C12N 2310/336 (2013.01); C12N 2310/351 (2013.01); C12N 2310/3515 (2013.01); C12N 2330/30 (2013.01);
Abstract

Recent advancements in LNA oligonucleotides include the use of amine linkers to link an LNA antisense oligonucleotide to a conjugate group. For example please see WO2014/I18267. The present invention originates from the identification of a problem when de-protecting LNA oligonucleotides which comprise an aliphatic amine group and DMF protected LNA G nucleoside, which results in the production of a +28 Da impurity. This problem is solved by using acyl protection groups on the exocyclic nitrogen of the LNA-G residue, rather than the standard DMF protection group.


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