The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 28, 2023

Filed:

Apr. 23, 2021
Applicant:

Inscripta, Inc., Boulder, CO (US);

Inventors:

Phillip Belgrader, Pleasanton, CA (US);

Nathan Bade, Boulder, CO (US);

Christian Siltanen, Boulder, CO (US);

Aamir Mir, Boulder, CO (US);

Xi-Jun Chen, Boulder, CO (US);

Janine Mok, Boulder, CO (US);

Burak Dura, Boulder, CO (US);

Bruce Chabansky, Boulder, CO (US);

David Stumbo, Boulder, CO (US);

Eric Smith, Boulder, CO (US);

Jorge Bernate, Boulder, CO (US);

Assignee:

Inscripta, Inc., Boulder, CO (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/11 (2006.01); C12N 15/10 (2006.01); C12M 1/34 (2006.01); C12M 1/26 (2006.01); C12N 15/113 (2010.01); C12N 15/86 (2006.01); C12M 3/00 (2006.01); C12M 3/04 (2006.01); C12M 1/00 (2006.01); C12N 5/074 (2010.01); C12N 15/88 (2006.01); C12N 15/90 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); C12M 1/32 (2006.01); C12M 3/06 (2006.01); C12N 9/22 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1082 (2013.01); B01L 3/502761 (2013.01); B01L 7/00 (2013.01); C12M 23/12 (2013.01); C12M 23/16 (2013.01); C12M 23/42 (2013.01); C12M 23/50 (2013.01); C12M 27/10 (2013.01); C12M 29/04 (2013.01); C12M 33/14 (2013.01); C12M 41/36 (2013.01); C12M 43/00 (2013.01); C12M 47/02 (2013.01); C12M 47/04 (2013.01); C12N 5/0696 (2013.01); C12N 15/1065 (2013.01); C12N 15/1068 (2013.01); C12N 15/11 (2013.01); C12N 15/113 (2013.01); C12N 15/86 (2013.01); C12N 15/88 (2013.01); C12N 15/907 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/123 (2013.01); B01L 2300/161 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/0421 (2013.01); B01L 2400/0424 (2013.01); C12N 9/22 (2013.01); C12N 2310/20 (2017.05); C12N 2510/00 (2013.01); C12N 2740/10011 (2013.01); C12N 2740/15011 (2013.01); C12N 2750/14111 (2013.01); C12N 2800/80 (2013.01);
Abstract

This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells. The disclosed compositions and method entail making 'reagent bundles' comprising many (hundreds of thousands to millions) clonal copies of an editing cassette and delivering or co-localizing the reagent bundles with live mammalian cells such that the editing cassettes edit the cells and the edited cells continue to grow.


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