The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 28, 2023
Filed:
Aug. 22, 2019
Applicant:
New England Biolabs, Inc., Ipswich, MA (US);
Inventors:
Paul A. Koetsier, Ipswich, MA (US);
Barbara W. Taron, Essex, MA (US);
Eric J. Cantor, Topsfield, MA (US);
Assignee:
New England Biolabs, Inc., Ipswich, MA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); B01L 3/00 (2006.01); B01L 9/06 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1006 (2013.01); B01L 3/5082 (2013.01); B01L 9/06 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0609 (2013.01); B01L 2300/12 (2013.01); B01L 2300/16 (2013.01);
Abstract
Provided herein is a method for isolating high molecular weight (HMW) DNA using beads that are at least 200 μm in diameter that utilizes a device for retaining the beads and where the purified DNA eluant exits the device without shearing the HMW DNA. In some embodiments, the method comprises precipitating the DNA onto the beads, washing the beads in the device, and then eluting the DNA from the beads therein while substantially avoiding shear. Compositions and kits for practicing the method are also provided.