The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 28, 2023

Filed:

Oct. 23, 2018
Applicant:

The Charles Stark Draper Laboratory, Inc., Cambridge, MA (US);

Inventors:

Vishal Tandon, Roxbury Crossing, MA (US);

Charles A. Lissandrello, Newtonville, MA (US);

Ryan A. Dubay, Cambridge, MA (US);

Rebecca Christianson, Cambridge, MA (US);

Jenna Leigh Balestrini, Boston, MA (US);

Peter Hsi, Waltham, MA (US);

Jason Fiering, Boston, MA (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12M 1/42 (2006.01); A61K 9/00 (2006.01); C12N 15/87 (2006.01); C12M 3/06 (2006.01); B01L 3/00 (2006.01); A61N 1/32 (2006.01); B82Y 5/00 (2011.01); A61N 7/00 (2006.01);
U.S. Cl.
CPC ...
C12M 35/02 (2013.01); A61K 9/0009 (2013.01); A61N 1/327 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502761 (2013.01); C12M 23/16 (2013.01); C12M 35/04 (2013.01); C12N 15/87 (2013.01); A61N 7/00 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0816 (2013.01); B01L 2400/0436 (2013.01); B82Y 5/00 (2013.01);
Abstract

Transfer of genetic and other materials to cells is conducted in a hands-free, automated and continuous process that includes flowing the cells between electroporation electrodes to facilitate delivery of a payload into the cells, while acoustophoretically focusing the cells. Also described is a control method for the acoustophoretic focusing of cells that includes detecting locations of cells flowing through a channel, such as with an image analytics system, and modulating a drive signal to an acoustic transducer to change the locations of the cells flowing in the channel. Finally, an electroporation driver module is described that uses a digital to analog converter for generating an electroporation waveform and an amplifier for amplifying the electroporation waveform for application to electroporation electrodes.


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