The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 21, 2023
Filed:
Jul. 30, 2021
Shandong Institute for Food and Drug Control, Shandong, CN;
Shandong University, Shandong, CN;
Avanc Pharmaceutical Co., Ltd, Liaoning, CN;
Feng Shi, Shandong, CN;
Liping Gong, Shandong, CN;
Ruiqing Xian, Shandong, CN;
Lianli Chi, Shandong, CN;
Weijian Wang, Shandong, CN;
Baojian Hang, Shandong, CN;
Congcong Wang, Shandong, CN;
Pengfei You, Shandong, CN;
Hongming Du, Liaoning, CN;
Shandong Institute for Food and Drug Control, Shandong, CN;
Shandong University, Shandong, CN;
Avanc Pharmaceutical Co., Ltd, Liaoning, CN;
Abstract
The present invention relates to the technical field of chemical analysis and quantitative detection, in particular to a quantitative detection method for snake venom thrombin-like enzyme (SVTLE) from. The quantitative detection method for the SVTLE includes the following steps of taking a reference substance of marker peptide for the SVTLE fromwith an amino acid sequence of LDSPVSNSAHIAPLSLPSSAPSVGSVCR, and preparing a series of reference solutions with different concentrations; adding the reference solutions in test solutions respectively for enzymolysis, and then taking a supernatant after enzymolysis as a series of solutions to be detected; and adding the solutions to be detected in a liquid chromatogram-mass spectrometer, and then selecting a qualitative ion pair and a quantitative ion pair to detect contents of marker peptide in the solutions to be detected.