The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 21, 2023

Filed:

Sep. 20, 2018
Applicant:

The Charles Stark Draper Laboratory, Inc., Cambridge, MA (US);

Inventors:

Kenneth T. Kotz, Newton, MA (US);

Bryan D. Teece, Boston, MA (US);

James Gillett Truslow, Boston, MA (US);

Nathan Francis Moore, Canton, MA (US);

Jeffrey T. Borenstein, Holliston, MA (US);

Vishal Tandon, Somerville, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/02 (2006.01); C12M 1/12 (2006.01); C12N 15/87 (2006.01); C12M 1/00 (2006.01); C12N 15/86 (2006.01); C12M 3/06 (2006.01); C12M 1/40 (2006.01); C12M 1/34 (2006.01); A61K 48/00 (2006.01);
U.S. Cl.
CPC ...
C12M 27/00 (2013.01); C12M 21/18 (2013.01); C12M 23/16 (2013.01); C12M 23/40 (2013.01); C12M 25/02 (2013.01); C12M 25/06 (2013.01); C12M 27/18 (2013.01); C12M 29/00 (2013.01); C12M 29/04 (2013.01); C12M 29/10 (2013.01); C12M 29/18 (2013.01); C12M 41/12 (2013.01); C12M 41/46 (2013.01); C12M 47/04 (2013.01); C12N 15/86 (2013.01); C12N 15/87 (2013.01); A61K 48/0083 (2013.01); C12N 2740/16043 (2013.01); C12N 2750/10043 (2013.01);
Abstract

A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.


Find Patent Forward Citations

Loading…