The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 14, 2023

Filed:

Aug. 15, 2019
Applicants:

Helmholtz Zentrum München Deutsches Forschungszentrum Für Gesundheit Und Umwelt (Gmbh), Neuherberg, DE;

Technische Universität München, Munich, DE;

Inventors:

Wen-Min Chou, Miaoli County, TW;

Ulrike Protzer-Knolle, Munich, DE;

Martin Mueck-Haeusl, Grasbrunn, DE;

Chunkyu Ko, Munich, DE;

Jochen Wettengel, Wertheim, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/63 (2006.01); C12N 15/74 (2006.01); C07H 21/04 (2006.01); A61K 48/00 (2006.01); C12N 15/10 (2006.01); C12N 15/86 (2006.01); C07K 14/47 (2006.01); G01N 33/50 (2006.01); C12N 15/85 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1086 (2013.01); C07K 14/4731 (2013.01); C12N 15/86 (2013.01); C12Y 207/07 (2013.01); G01N 33/5067 (2013.01); C07H 21/04 (2013.01); C12N 15/63 (2013.01); C12N 2015/859 (2013.01); C12N 2800/40 (2013.01);
Abstract

The present invention discloses a method for assessing the capacity of a substance to treat or prevent hepadnavirus infection. A reporter virus carrying genetic information for a first fragment of a recombinase and a reporter cell expressing a second fragment of the recombinase are used. When the reporter virus infects the reporter cell, the two fragments of the recombinase associate and excise a stop cassette that is flanked by two recombination sites and blocks the expression of a reporter gene. Accordingly, the present invention relates to a method of assessing the capacity of a substance to treat or prevent hepadnavirus infection, a hepadnavirus comprising a nucleic acid encoding a first fragment of a recombinase and a mammalian hepatocyte or hepatoma cell comprising a nucleic acid encoding a second fragment of a recombinase and a nucleic acid comprising a stop cassette flanked by two recombination sites fused to a reporter gene.


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