The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 14, 2023

Filed:

Jan. 15, 2018
Applicants:

Kaneka Corporation, Osaka, JP;

Tokyo University of Science Foundation, Tokyo, JP;

Inventors:

Tatsuya Moutai, Hyogo, JP;

Tomoyuki Nakaishi, Hyogo, JP;

Hiroshi Kita, Hyogo, JP;

Mitsuaki Kitano, Hyogo, JP;

Daisuke Kitamura, Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); C07K 16/28 (2006.01); C12N 5/0781 (2010.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
C07K 16/2878 (2013.01); A61K 39/0011 (2013.01); C07K 16/2875 (2013.01); C12N 5/0635 (2013.01); C07K 2317/14 (2013.01); C12N 2501/2302 (2013.01); C12N 2501/232 (2013.01); C12N 2501/2304 (2013.01); C12N 2501/2312 (2013.01); C12N 2501/2321 (2013.01); C12N 2501/2324 (2013.01); C12N 2501/2327 (2013.01); C12N 2501/25 (2013.01); C12N 2501/52 (2013.01);
Abstract

It is an object of the present invention to provide a method for efficiently producing a B cell population comprising B cells that recognize a specific antigen. According to the present invention, provided is a method for producing a B cell population, comprising: a step (c) of culturing a cell population comprising B cells together with a specific antigen in the absence of IL-21, in the absence of IL-4, and in the presence of a cytokine other than IL-21 and IL-4, while giving stimulation mediated by CD40 and a BAFF receptor to the cells; and a step (d) of culturing the cell population comprising B cells, while giving stimulation mediated by has to the cells, so as to obtain a B cell population comprising B cells that recognize the specific antigen.


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