The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 07, 2023

Filed:

Dec. 19, 2017
Applicant:

Precision Nanosystems Ulc, Vancouver, CA;

Inventors:

Anitha Thomas, New Westminster, CA;

Rebecca Anne Grace De Souza, Vancouver, CA;

Eric Ouellet, New Westminster, CA;

Grace Tharmini Tharmarajah, Toronto, CA;

Jagbir Singh, Vancouver, CA;

Shyam Madhusudan Garg, Richmond, CA;

Assignee:

Precision NanoSystems ULC, Vancouver, CA;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/88 (2006.01); A61K 9/14 (2006.01); A61K 9/51 (2006.01); A61K 47/14 (2017.01); A61K 47/18 (2017.01); C12N 5/0793 (2010.01); C12N 15/11 (2006.01); C12N 15/113 (2010.01); C12N 15/85 (2006.01); C07F 9/10 (2006.01); C12N 15/87 (2006.01); C07J 41/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/88 (2013.01); A61K 9/14 (2013.01); A61K 9/5123 (2013.01); A61K 47/14 (2013.01); A61K 47/18 (2013.01); C07F 9/106 (2013.01); C12N 5/0619 (2013.01); C12N 15/111 (2013.01); C12N 15/113 (2013.01); C12N 15/85 (2013.01); C12N 15/87 (2013.01); C07J 41/0055 (2013.01); C12N 2310/11 (2013.01); C12N 2310/12 (2013.01); C12N 2310/14 (2013.01); C12N 2310/141 (2013.01); C12N 2310/16 (2013.01); C12N 2310/531 (2013.01); C12N 2320/32 (2013.01); C12N 2500/35 (2013.01); C12N 2500/36 (2013.01); C12N 2500/50 (2013.01);
Abstract

A transfection reagent composition comprising: 30-60 MOL. % of a cationic lipid, or a pharmaceutical acceptable salt thereof; 10-60 MOL % of a structural lipid; 10-20 MOL % of a triglyceride; and 0.1 to about 10 MOL. % of a stabilizing agent, is provided. The transfection agent is effective in transfecting cells, particularly neurons, with siRNA, mRNA and plasmid nucleic acid, Sand maintaining viability of the cells as well as activity of the delivered nucleic acid.


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