The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 07, 2023

Filed:

Dec. 06, 2018
Applicant:

University of Cape Town, Cape Town, ZA;

Inventors:

Eden Padayachee, Cape Town, ZA;

Fleury Augustin Nsole Biteghe, Cape Town, ZA;

Olusiji Alex Akinrinmade, Cape Town, ZA;

Sandra Jordaan, Cape Town, ZA;

Stefan Barth, Green Point, ZA;

Assignee:

UNIVERSITY OF CAPE TOWN, Cape Town, ZA;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/47 (2006.01); C12N 9/10 (2006.01); C12N 15/62 (2006.01); G01N 33/569 (2006.01); G01N 33/573 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1007 (2013.01); C07K 14/47 (2013.01); C12N 15/62 (2013.01); C12Y 201/01063 (2013.01); G01N 33/56966 (2013.01); G01N 33/573 (2013.01); C07K 2319/21 (2013.01); C07K 2319/50 (2013.01); G01N 2333/91017 (2013.01); G01N 2510/00 (2013.01);
Abstract

The present invention relates to fusion proteins which are capable of binding to phosphatidylserine comprising a phosphatidylserene binding ligand and a modified O6-alkylguanine-DNA alkyltransferase which is capable of autoconjugation to an O6-benzylguanine-modified label, the fusion proteins being capable of binding to phosphatidylserine on the surface of a cell undergoing apoptosis. The invention also relates to recombinant polypeptide precursors of the fusion proteins which comprise a secretion leader sequence, purification tag, protease cleavage site and the fusion protein. Also included in the scope of the invention are nucleic acids encoding the recombinant polypeptide precursor, vectors comprising the nucleic acids, host cells comprising the vectors, methods of production of the fusion proteins, kits and assays for detecting apoptosis.


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