The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 31, 2023
Filed:
Apr. 18, 2019
Applicant:
Jiangnan University, Wuxi, CN;
Inventors:
Assignee:
Jiangnan University, Wuxi, CN;
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/10 (2006.01); C12N 15/70 (2006.01); C12N 15/75 (2006.01); A23L 23/00 (2016.01); A23L 33/13 (2016.01); A23L 29/00 (2016.01);
U.S. Cl.
CPC ...
C12N 9/1048 (2013.01); A23L 29/06 (2016.08); A23L 33/13 (2016.08); C12N 15/70 (2013.01); C12N 15/75 (2013.01); C12Y 204/01019 (2013.01); A23V 2002/00 (2013.01); C12N 2800/101 (2013.01);
Abstract
The present invention discloses a mutant of cyclodextrin glycosyltransferase and belongs to the fields of gene engineering and enzyme engineering. According to the present invention, a mutant having higher disproportionation activity of cyclodextrin glycosyltransferase is obtained by mutating the cyclodextrin glycosyltransferase. The disproportionation activity of enzymes of mutants V6D, S90G, T168A, T171A, T383A, G608A, and V6D/S90G/T168A/T171A/T383A/G608A, is respectively 1.89 times, 1.21 times, 1.21 times, 1.22 times, 1.32 times, 2.03 times, and 3.16 times that of the wild type enzyme in shake flask fermentations.