The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 31, 2023

Filed:

Dec. 29, 2016
Applicants:

Lab Skin Creations, Lyons, FR;

Universite Claude Bernard Lyon 1, Villeurbanne, FR;

Centre National DE LA Recherche Scientifique, Paris, FR;

Institut National Des Sciences Appliquees DE Lyon, Villeurbanne, FR;

Ecole Superieure DE Chimie, Physique, Electronique DE Lyon, Villeurbanne, FR;

Inventors:

Christophe Marquette, Villeurbanne, FR;

Léa Pourchet, Lyons, FR;

Amélie Thepot, Lyons, FR;

Morgan Dos Santos, Saint Verand, FR;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); B33Y 70/00 (2020.01); B33Y 80/00 (2015.01); G01N 33/50 (2006.01); B33Y 10/00 (2015.01); A61L 27/38 (2006.01); C12N 5/077 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0698 (2013.01); A61L 27/3843 (2013.01); B33Y 10/00 (2014.12); B33Y 70/00 (2014.12); B33Y 80/00 (2014.12); C12N 5/0629 (2013.01); C12N 5/0656 (2013.01); G01N 33/50 (2013.01); C12N 2500/38 (2013.01); C12N 2501/11 (2013.01); C12N 2501/33 (2013.01); C12N 2501/39 (2013.01); C12N 2501/395 (2013.01); C12N 2501/805 (2013.01); C12N 2502/091 (2013.01); C12N 2502/092 (2013.01); C12N 2502/094 (2013.01); C12N 2502/097 (2013.01); C12N 2502/1305 (2013.01); C12N 2502/1323 (2013.01); C12N 2503/06 (2013.01); C12N 2533/30 (2013.01); C12N 2533/52 (2013.01); C12N 2533/54 (2013.01); C12N 2533/56 (2013.01); C12N 2533/74 (2013.01);
Abstract

The invention relates to a method for manufacturing a bio-ink by additive deposition, which comprises supplying: a first solution including between 5 and 40 wt. % gelatin; a second solution including between 15 and 35.wt. % alginate; a third solution including between 1 and 15 wt. % fibrinogen, and optionally living cells in suspension; and creating a mixture including: around 35 to 65 vol. % of the first solution; around 15 to 35 vol. % of the second solution; and around 15 to 35 vol. % of the third solution, said proportions being selected so that they add up to 100%. Said bio-ink allows the additive deposition of objects that can be polymerised by means of a solution including calcium ions and thrombin. Said objects can be incubated and can be used as a substitute for body tissue, for example (with added fibroblasts) as skin substitute.


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