The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 24, 2023

Filed:

Jan. 02, 2018
Applicants:

Quidel Corporation, San Diego, CA (US);

Guild Associates, Inc., Dublin, OH (US);

Inventors:

Alexander Solomon Belenky, San Diego, CA (US);

David A. Schofield, Hollywood, SC (US);

Assignees:

Quidel Corporation, San Diego, CA (US);

Guild Associates, Inc., Dublin, OH (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/04 (2006.01); C12Q 1/18 (2006.01); C12Q 1/689 (2018.01); G01N 33/569 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/04 (2013.01); C12Q 1/18 (2013.01); C12Q 1/689 (2013.01); G01N 33/56911 (2013.01); G01N 2333/01 (2013.01); G01N 2333/195 (2013.01); Y02A 50/30 (2018.01);
Abstract

Methods for determining the susceptibility or resistance of bacteria to antibiotic agents are provided. In one embodiment, the methods include culturing the bacteria in the presence or absence or the antimicrobial agent to generate a primary culture which is then cultured in the presence or absence of transforming phages. The recombinant phages are specific to the bacteria and comprise a heterologous marker (e.g., a nucleic acid that is expressible as a detectable product such as an RNA or a protein). The susceptibility or resistance of the bacteria to the antimicrobial agent may be determined by assaying the culture for the presence or absence of the heterologous marker, wherein a reduction in the level or activity of the marker in the culture compared to the level or activity of the marker in a comparative culture indicates that the bacteria is sensitive to the antibiotic agent.


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