The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 24, 2023

Filed:

Sep. 16, 2019
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Abraham P. Lee, Irvine, CA (US);

Roger Shih, Irvine, CA (US);

Wei-Feng Fang, Irvine, CA (US);

Naiqing Zhang, San Diego, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C12N 5/00 (2006.01); C12N 11/04 (2006.01); B01F 33/3011 (2022.01); G01N 1/38 (2006.01); B01F 23/41 (2022.01); B01F 31/00 (2022.01); B01F 31/65 (2022.01);
U.S. Cl.
CPC ...
B01L 3/502784 (2013.01); B01F 23/41 (2022.01); B01F 31/57 (2022.01); B01F 31/65 (2022.01); B01F 33/3011 (2022.01); C12N 5/0012 (2013.01); C12N 11/04 (2013.01); B01L 2200/0636 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0673 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0439 (2013.01); B01L 2400/0487 (2013.01); G01N 1/38 (2013.01);
Abstract

A method of encapsulating a solid sample in a droplet, the method including flowing a continuous phase through a first fluid channel at a first flow rate; flowing a dispersed phase through a second fluid channel at a second flow rate, the dispersed phase including a plurality of particles, cells or beads; trapping the plurality of particles, cells or beads in a mixing region that receives the dispersed phase and the continuous phase; and reducing the first flow rate to encapsulate the trapped particles, cells or beads in droplets of the dispersed phase generated when the dispersed phase and the continuous phase exit the mixing region through an orifice.


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