The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 17, 2023

Filed:

May. 18, 2018
Applicant:

Commissariat a L'energie Atomique ET Aux Energies Alternatives, Paris, FR;

Inventors:

Thomas Bordy, Grenoble, FR;

Olivier Cioni, Grenoble, FR;

Camille Deforceville, Anglars, FR;

Ondrej Mandula, Grenoble, FR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 15/14 (2006.01); C12Q 1/06 (2006.01); G02B 21/36 (2006.01); G03H 1/00 (2006.01); G03H 1/04 (2006.01); G01N 15/10 (2006.01);
U.S. Cl.
CPC ...
G01N 15/1434 (2013.01); C12Q 1/06 (2013.01); G01N 15/1475 (2013.01); G02B 21/367 (2013.01); G03H 1/0005 (2013.01); G03H 1/0443 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1454 (2013.01); G03H 2001/005 (2013.01); G03H 2001/0447 (2013.01); G03H 2226/02 (2013.01); G03H 2226/11 (2013.01);
Abstract

A method for analyzing microorganisms arranged in a sample is provided, the sample including a viability marker to modify an optical property of the microorganisms in different ways depending on whether they are dead or alive, the method including illumination of the sample and acquisition of an image of the latter by an image sensor, the image sensor then being exposed to an exposure light wave; determining positions of different microorganisms from the acquired image; applying a propagation operator to calculate at least one characteristic value of the exposure light wave at each radial position and at a plurality of distances from the detection plane representing a change in the characteristic value between the image sensor and the sample; and identifying living microorganisms according to each profile.


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