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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11549950 B1

PDFFull Text
Date of Patent:
Jan. 10, 2023

Filed:

Jul. 30, 2018

Cryptic metabolites and method for activating silent biosynthetic gene clusters in diverse microorganisms

Applicant:

The Trustees of Princeton University, Princeton, NJ (US);

Inventors:

Mohammad R. Seyedsayamdost, Princeton, NJ (US);

Fei Xu, Princeton, NJ (US);

Yihan Wu, Princeton, NJ (US);

Leah Bushin, Princeton, NJ (US);

Katherine Davis, Princeton, NJ (US);

Assignee:

THE TRUSTEES OF PRINCETON UNIVERSITY;

Attorney:

Meagher Emanuel Laks Goldberg & Liao, LLP

Primary Examiner:

Iqbal H Chowdhury

Int. Cl.
CPC ...
G01N 33/68 (2006.01); C07K 9/00 (2006.01); C12N 1/20 (2006.01); C12N 1/38 (2006.01); C12P 19/56 (2006.01); C12P 21/00 (2006.01); C12Q 1/18 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6848 (2013.01); C07K 9/008 (2013.01); C12N 1/20 (2013.01); C12N 1/38 (2013.01); C12P 19/56 (2013.01); C12P 21/005 (2013.01); C12Q 1/18 (2013.01);
Abstract

Disclosed herein is a rapid genetics-free method for eliciting and detecting cryptic metabolites using an imaging mass spectrometry-based approach. An organism of choice is challenged with elicitors from a small molecule library. The molecules elicited are then imaged by mass spec, which allows for rapid identification of cryptic metabolites. These are then isolated and characterized. Employing the disclosed approach activated production of cryptic glycopeptides from an actinomycete bacterium. The molecules that result, the keratinimicins and keratinicyclins, are metabolites with important structural features. At least two of these, keratinimicins B and C, are highly bioactive against several pathogenic strains. This approach will allow for rapid activation and identification of cryptic metabolites from diverse microorganisms in the future.

Find Patent Forward Citations

Seyedsayamdost, Mohammad. (2023). Cryptic metabolites and method for activating silent biosynthetic gene clusters in diverse microorganisms (U.S. Patent No. 11549950). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/11549950

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