The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 10, 2023
Filed:
Mar. 28, 2017
Japan Tobacco Inc., Tokyo, JP;
Tokyo Metropolitan University, Tokyo, JP;
Riken, Wako, JP;
Norio Kato, Shizuoka, JP;
Takashi Okamoto, Tokyo, JP;
Takatoshi Kiba, Saitama, JP;
Erika Toda, Saitama, JP;
RIKEN, Wako, JP;
Abstract
The present invention relates to a method for introducing a substance into a plant. The method of the present invention comprises the steps of: obtaining an enzymatically treated and isolated fertilized egg cell by (1-i) isolating a fertilized egg cell from a plant tissue containing a fertilized egg cell, and then treating the fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, (1-ii) treating a plant tissue containing a fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and then isolating the fertilized egg cell that has been enzymatically treated, (1-iii) treating a plant tissue containing a fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and simultaneously isolating the fertilized egg cell that has been enzymatically treated, (1-iv) isolating an egg cell and a sperm cell from a plant to produce a fertilized egg by fusing the cells, and then treating the fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, or (1-v) treating a plant tissue containing an egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and then isolating the egg cell that has been enzymatically treated, and further fusing the egg cell with an isolated sperm cell; and (2) introducing a substance selected from the group consisting of nucleic acids, proteins, and peptides into the resultant enzymatically treated and isolated fertilized egg cell.