The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 03, 2023
Filed:
Jul. 27, 2021
Applicant:
Roche Diagnostics Operations, Inc., Indianapolis, IN (US);
Inventors:
Thomas Greiner-Stoeffele, Soemmerda, DE;
Stefan Schoenert, Leipzig, DE;
Assignee:
Roche Diagnostics Operations, Inc., Indianpolis, IN (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/54 (2006.01); C12N 15/31 (2006.01); C12N 15/57 (2006.01); C12N 1/19 (2006.01); C12N 9/52 (2006.01); C07K 14/195 (2006.01); C12N 5/071 (2010.01);
U.S. Cl.
CPC ...
C12N 9/52 (2013.01); C07K 14/195 (2013.01); C12N 5/0602 (2013.01); C12Y 304/24 (2013.01); C12Y 304/24028 (2013.01); C12N 2509/00 (2013.01);
Abstract
The present disclosure provides the sequence of apreproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.