The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 03, 2023

Filed:

Aug. 14, 2018
Applicants:

Sumitomo Chemical Company, Limited, Tokyo, JP;

Riken, Wako, JP;

Inventors:

Tokushige Nakano, Osaka, JP;

Satoshi Ando, Osaka, JP;

Yoshiki Sasai, Wako, JP;

Mototsugu Eiraku, Wako, JP;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/079 (2010.01); A61K 35/30 (2015.01); A61L 27/36 (2006.01); A61L 27/38 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0621 (2013.01); A61K 35/30 (2013.01); A61L 27/3604 (2013.01); A61L 27/3895 (2013.01); C12N 2500/90 (2013.01); C12N 2501/41 (2013.01); C12N 2501/415 (2013.01); C12N 2501/727 (2013.01); C12N 2506/02 (2013.01); C12N 2506/08 (2013.01);
Abstract

The invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure by culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more of the tissue in a serum-free medium or serum-containing medium, each containing a substance acting on the Wnt signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the 'cell aggregate in which a RPE65 gene expressing cell does not appear' thus obtained in a serum-free medium or serum-containing medium, each not containing a substance acting on the Wnt signal pathway and so on.


Find Patent Forward Citations

Loading…