The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 03, 2023

Filed:

Feb. 07, 2018
Applicant:

Tosoh Corporation, Yamaguchi, JP;

Inventors:

Yosuke Terao, Kanagawa, JP;

Yoshiharu Asaoka, Kanagawa, JP;

Ryoko Otake, Kanagawa, JP;

Satoshi Endo, Kanagawa, JP;

Naoki Yamanaka, Kanagawa, JP;

Yukie Yamamoto, Kanagawa, JP;

Seigo Oe, Kanagawa, JP;

Assignee:

TOSOH CORPORATION, Yamaguchi, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 1/22 (2006.01); C07K 17/00 (2006.01); C07K 14/00 (2006.01); C07K 16/00 (2006.01);
U.S. Cl.
CPC ...
C07K 1/22 (2013.01); C07K 14/00 (2013.01); C07K 16/00 (2013.01);
Abstract

The present application addresses the problem of providing an Fc-binding protein having an improved antibody separation ability. The present application also addresses the problem of providing a high-accuracy antibody separation method using an insoluble carrier having the protein immobilized thereon. The problems can be solved by: an Fc-binding protein in which at least an amino acid substitution at a specific position therein occurs and which has reduced affinity for an antibody; and an antibody separation method including allowing an equilibration buffer solution to pass through a column in which an insoluble carrier having the protein immobilized thereon is filled to equilibrate the column, adding a solution containing an antibody to cause the adsorption of the antibody onto the carrier, and eluting the antibody adsorbed on the carrier using an elution solution.


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