The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 27, 2022

Filed:

Jan. 06, 2021
Applicant:

Sharp Life Science (Eu) Limited, Oxford, GB;

Inventors:

Benjamin James Hadwen, Oxford, GB;

Adrian Marc Simon Jacobs, Oxford, GB;

Jason Roderick Hector, Oxford, GB;

Michael James Brownlow, Oxfordshire, GB;

Masahiro Adachi, Osaka, JP;

Alison Mary Skinner, Mansfield, MA (US);

Mark Childs, Formby Merseyside, GB;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/49 (2006.01); C12Q 1/6844 (2018.01); B01L 3/00 (2006.01); G01N 33/579 (2006.01); C12Q 1/6816 (2018.01); G01N 30/60 (2006.01); G01N 30/88 (2006.01);
U.S. Cl.
CPC ...
G01N 33/4905 (2013.01); B01L 3/502792 (2013.01); C12Q 1/6844 (2013.01); B01L 2200/14 (2013.01); B01L 2200/148 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/1822 (2013.01); B01L 2400/0427 (2013.01); B01L 2400/0677 (2013.01); C12Q 1/6816 (2013.01); G01N 30/6095 (2013.01); G01N 33/579 (2013.01); G01N 2030/8813 (2013.01);
Abstract

A method of determining the result of an assay in a microfluidic device includes the steps of: dispensing a sample droplet onto a first portion of an electrode array of the microfluidic device; dispensing a reagent droplet onto a second portion of the electrode array of the microfluidic device; controlling actuation voltages applied to the electrode array to mix the sample droplet and the reagent droplet into a product droplet; sensing a dynamic property of the product droplet; and determining an assay of the sample droplet based on the sensed dynamic property. The dynamic property is a physical property of the product droplet that influences a transport property of the product droplet on the electrode array. Example dynamic properties of the product droplet include the moveable state, split-able state, and viscosity based on droplet properties. The method may be used to perform an amoebocyte lysate (LAL) assay.


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