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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11535878 B1

PDFFull Text
Date of Patent:
Dec. 27, 2022

Filed:

Dec. 26, 2017

In vivo synthesis of sialylated compounds

Applicant:

Inbiose N.v., Ghent, BE;

Inventors:

Joeri Beauprez, Bredene, BE;

Pieter Coussement, Gentebrugge, BE;

Dries Van Herpe, Wondelgem, BE;

Gert Peters, Ghent, BE;

Annelies Vercauteren, Eke, BE;

Assignee:

INBIOSE N.V., Ghent, BE;

Attorney:

Barbara E. Johnson, Esq.

Primary Examiner:

Tekchand Saidha

Assistant Examiner:

Mohammad Y Meah

Int. Cl.
CPC ...
C12P 19/16 (2006.01); C12P 19/04 (2006.01); C12N 1/20 (2006.01); C12P 19/18 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 9/16 (2006.01); C12N 9/80 (2006.01); C12N 9/88 (2006.01); C12N 9/90 (2006.01); C12N 15/52 (2006.01); C12P 19/26 (2006.01);
U.S. Cl.
CPC ...
C12P 19/18 (2013.01); C12N 9/1029 (2013.01); C12N 9/1085 (2013.01); C12N 9/1205 (2013.01); C12N 9/16 (2013.01); C12N 9/80 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 15/52 (2013.01); C12P 19/26 (2013.01);
Abstract

The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of fermentation of metabolically engineered microorganisms. The present invention describes engineered microorganisms able to synthesize sialylated compounds via an intracellular biosynthesis route. These microorganisms can dephosphorylate N-acetylglucosamine-6-phosphate to N-acetylglucosamine and convert the N-acetylglucosamine to N-acetylmannosamine. These microorganisms also have the ability to convert N-acetylmannosamine to N-acetyl-neuraminate. Furthermore, the present invention provides a method for the large scale in vivo synthesis of sialylated compounds, by culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as, but not limited to lactose, lactoNbiose, N-acetyllactosamine and/or an aglycon, wherein said microorganism intracellularly dephosphorylates N-acetylglucosamine-6-phosphate to N-acetylglucosamine, converts N-acetylglucosamine to N-acetylmannosamine and convert the latter further to N-acetyl-neuraminate.

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