The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 27, 2022

Filed:

Dec. 16, 2016
Applicants:

Shanghai Jiao Tong University, Shanghai, CN;

Jecho Laboratories Inc., Frederick, MD (US);

Jecho Biopharmaceuticals CO Ltd., Tianjin, CN;

Inventors:

Lei Han, Shanghai, CN;

Jianwei Zhu, Shanghai, CN;

Junsheng Chen, Shanghai, CN;

Kai Ding, Shanghai, CN;

Yueqing Xie, Shanghai, CN;

Hua Jiang, Shanghai, CN;

Huili Lu, Shanghai, CN;

Baohong Zhang, Shanghai, CN;

Lei Zhang, Shanghai, CN;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/62 (2006.01); C07K 16/28 (2006.01); C07K 16/46 (2006.01); C07K 16/06 (2006.01);
U.S. Cl.
CPC ...
C07K 16/2863 (2013.01); C07K 16/065 (2013.01); C07K 16/46 (2013.01); C12N 15/62 (2013.01); C07K 2317/31 (2013.01); C07K 2317/35 (2013.01); C07K 2317/51 (2013.01); C07K 2317/524 (2013.01); C07K 2317/92 (2013.01); C07K 2319/20 (2013.01); C07K 2319/92 (2013.01);
Abstract

The present disclosure discloses a method for expressing and preparing a bivalent bispecific antibody. In the present disclosure, each portion of a bivalent bispecific antibody and an immune hybrid protein thereof is respectively expressed in a suitable prokaryotic or eukaryotic cell system, separated and purified by high-performance affinity chromatography, and then spliced in vitro by trans-splicing reaction mediated by an intein, to prepare the bivalent specific antibody and an immune hybrid protein thereof.


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