The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 20, 2022
Filed:
Dec. 13, 2021
Ningbo University, Zhejiang, CN;
Xingfeng Shao, Zhejiang, CN;
Yingying Wei, Zhejiang, CN;
Yihui Mao, Zhejiang, CN;
Yi Chen, Zhejiang, CN;
Shu Jiang, Zhejiang, CN;
NINGBO UNIVERSITY, Zhejiang, CN;
Abstract
The present invention discloses apolygalacturonase-inhibiting protein 1 (PpPGIP1) gene, and a cloning method and use thereof. Thepolygalacturonase-inhibiting protein 1 PpPGIP1 gene has a nucleotide sequence shown in SEQ ID NO: 1, and a protein encoded by thepolygalacturonase-inhibiting protein 1 (PpPGIP1) gene has an amino acid sequence shown in SEQ ID NO: 2. The cloning method includes the following steps: (1) extracting total RNA from a peach, and subjecting the total RNA to reverse transcription to obtain cDNA, which serves as a template; (2) designing primers based on the PpPGIP1 gene sequence; and (3) Polymerase Chain Reaction (PCR) amplification: conducting PCR amplification to obtain a PpPGIP1 gene amplification product.