The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 20, 2022

Filed:

Jun. 09, 2017
Applicant:

The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US);

Inventors:

Ryan T. Swoboda, Orange Park, FL (US);

Yuhong Cao, Palo Alto, CA (US);

Sergio Leal-Ortiz, Stanford, CA (US);

Stefanie Rothkoetter, Stanford, CA (US);

Nicholas A. Melosh, Menlo Park, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/00 (2006.01); B01L 3/00 (2006.01); C12M 1/12 (2006.01); C12N 5/00 (2006.01); C23C 16/40 (2006.01); C23C 16/455 (2006.01); C23C 16/56 (2006.01);
U.S. Cl.
CPC ...
C12M 29/16 (2013.01); B01L 3/508 (2013.01); C12M 25/02 (2013.01); C12M 25/10 (2013.01); C12N 5/0075 (2013.01); C23C 16/402 (2013.01); C23C 16/403 (2013.01); C23C 16/405 (2013.01); C23C 16/45553 (2013.01); C23C 16/45555 (2013.01); C23C 16/56 (2013.01); B01L 2300/0609 (2013.01); B01L 2300/0672 (2013.01); B01L 2300/0832 (2013.01); B01L 2300/12 (2013.01); B01L 2400/0415 (2013.01); C12N 2531/00 (2013.01); C12N 2533/00 (2013.01); C12N 2539/00 (2013.01);
Abstract

Described herein are nanostraw well insert apparatuses (e.g., devices and systems) that include nanotubes extending through and out of a membrane so that a material can pass through the membrane from a fluid reservoir depot and into a cell grown onto the nanotubes when electrical energy (e.g., electroporation energy) is applied. In particular, the device, systems and methods described herein may be adapted for cell growth viability and transfection efficiency (e.g., >70%). These apparatuses may be readily integratable into cell culturing processes for improved transfection efficiency, intracellular transport, and cell viability.


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