The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 13, 2022

Filed:

Mar. 23, 2021
Applicant:

Ajinomoto Co., Inc., Tokyo, JP;

Inventors:

Miwa Konishi, Kawasaki, JP;

Shohei Kajimoto, Kawasaki, JP;

Yusuke Hagiwara, Kawasaki, JP;

Yasuhiro Mihara, Kawasaki, JP;

Shogo Nakano, Shizuoka, JP;

Tomoharu Motoyama, Shizuoka, JP;

Sohei Ito, Shizuoka, JP;

Assignee:

AJINOMOTO CO., INC., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/00 (2006.01); C12N 15/11 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
C12N 9/93 (2013.01); C12N 15/11 (2013.01); C12P 19/34 (2013.01); C12N 2310/14 (2013.01);
Abstract

Ligase mutants of the following (1), (2), or (3): (1) a ligase mutant comprising an amino acid sequence showing 95% or more identity to the amino acid sequence of SEQ ID NO: 1, and having a nucleic acid-linking activity; (2) a ligase mutant comprising an amino acid sequence showing 90% or more identity to the amino acid sequence of SEQ ID NO: 2, and having a nucleic acid-linking activity; or (3) a ligase mutant comprising an amino acid sequence showing 97% or more identity to the amino acid sequence of SEQ ID NO: 3, and having a nucleic acid-linking activity, have excellent properties.


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